Universal Real-Time PCR-Based Assay for Lentiviral Titration
نویسندگان
چکیده
منابع مشابه
Universal Real-Time PCR-Based Assay for Lentiviral Titration
Lentiviral vectors are efficient vehicles for stable gene transfer in both dividing and non-dividing cells. This feature among others makes lentiviral vectors a powerful tool in molecular research. However, the use of lentiviruses in research studies and clinical trials requires a precise and validated titration method. In this study, we describe a qPCR-based approach for estimation of lentivir...
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lentiviral vectors (lvs) are useful vehicle for genetransfer to dividing and non-dividing cells and genetic manipulations. however, the use of lentiviruses in studies requires an accurate titration technique.quantitative real-time pcr (qpcr) is a sensitive technique for the indication and quantitation of retrovirals particles. in this study, we used the qpcr for lentiviral vector titeration. th...
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ژورنال
عنوان ژورنال: Molecular Biotechnology
سال: 2014
ISSN: 1073-6085,1559-0305
DOI: 10.1007/s12033-014-9815-4